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lamp1  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank lamp1
    Lamp1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+lamp1/anti-LAMP-1/pmc13126347-320-17-19
    Average 96 stars, based on 201 article reviews
    lamp1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Rational Design of New Biocompatible Near-Infrared Conjugated Polymer Nanoparticles for Biomedical Applications
    Article Snippet: .. The primary antibodies used were anti-LAMP1 and anti-CD63 (both from DSHB), and fluorescently labeled secondary antibodies were purchased from Jackson ImmunoResearch. ..

    Immunostaining:

    Article Title: Investigation of autophagy-activating molecules in a glia-specific Spinocerebellar ataxia type 1 model
    Article Snippet: .. Immunostaining of the cultures was performed using a protocol previously described using anti-LAMP1 (mouse, 1:100; #1D4B; DSHB),, and anti-p62/SQSTM1 (Merck SA, Darmstadt, Germany rabbit, 1:2000, P0067) as primary antibodies and anti-mouse Atto 550 (Merck SA, Darmstadt, Germany, 1:500; A21237), and anti-rabbit Alexa Fluor 633 (Merck SA, Darmstadt, Germany, 1:500; #A21070) as secondary ones. ..

    other:

    Article Title: Nexilin promotes calcium-dependent endo-lysosomal fission required for retrograde transport.
    Article Snippet: Both mediums were supplemented with 10% fetal bovine serum (FBS, Biological Industries), 100 U/ml penicillin and 100 mg/ml streptomycin (Sigma-Aldrich).

    Immunofluorescence:

    Article Title: Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation
    Article Snippet: .. Primary antibodies were Anti- Flag M2 HRP conjugated (A8592, Merck, 1:2000), anti-Vinculin (#V-9131, Merck, 1:5000), anti-phospho-AMPK (2535S, Cell signaling technology, 1:1000), anti-AMPK (2603S, Cell signaling technology, 1:1000), anti-phospho-p70 S6 kinase (9234S, Cell signaling technology, 1:1000), anti-p70 S6 kinase (9202, Cell signaling technology, 1:1000) and anti-LC3 (2775S, Cell signaling technology, 1:1000), anti-phospho-ATG13 (46329S, Cell signaling technology, 1:1000), anti-ATG13 (13273S, Cell signalling technology, 1:1000) anti-Lamp1 (H4A3, Developmental Studies Hybridoma Bank, University of Iowa, 1:100), anti-RyR (34 C) (Developmental Studies Hybridoma Bank, University of Iowa, 1:100 immunofluorescence, 1:1000 immunoblot), anti-Beclin-1 (sc-48341, Santa Cruz biotechnology 1:500), anti-SATB2 (ab51502, Abcam, 1:500), anti-ATP6v0a1 (ab237611, Abcam, 1:200 immunofluorescence, 1:1000 immunoblot) and anti-MAP2 (188002, Synaptic Systems, 1:500) anti-HA tag HRP conjugated (26183-HRP, Thermo Fisher Scientific, 1:1000), anti-FIP200 (10069-1-AP, proteintech,1:200) anti-WIPI2 (MCA5780GA, Biorad, 1:200). .. A rabbit polyclonal antibody targeting RyR2 was produced by LifeTein directed against the following epitope CKPEFNNHKDYAQEK of the mouse RyR2 sequence with a cysteine added to the N-terminal end (1:200) .

    Western Blot:

    Article Title: Inactive ryanodine receptors sustain lysosomal availability for autophagy by promoting ER-lysosomal contact site formation
    Article Snippet: .. Primary antibodies were Anti- Flag M2 HRP conjugated (A8592, Merck, 1:2000), anti-Vinculin (#V-9131, Merck, 1:5000), anti-phospho-AMPK (2535S, Cell signaling technology, 1:1000), anti-AMPK (2603S, Cell signaling technology, 1:1000), anti-phospho-p70 S6 kinase (9234S, Cell signaling technology, 1:1000), anti-p70 S6 kinase (9202, Cell signaling technology, 1:1000) and anti-LC3 (2775S, Cell signaling technology, 1:1000), anti-phospho-ATG13 (46329S, Cell signaling technology, 1:1000), anti-ATG13 (13273S, Cell signalling technology, 1:1000) anti-Lamp1 (H4A3, Developmental Studies Hybridoma Bank, University of Iowa, 1:100), anti-RyR (34 C) (Developmental Studies Hybridoma Bank, University of Iowa, 1:100 immunofluorescence, 1:1000 immunoblot), anti-Beclin-1 (sc-48341, Santa Cruz biotechnology 1:500), anti-SATB2 (ab51502, Abcam, 1:500), anti-ATP6v0a1 (ab237611, Abcam, 1:200 immunofluorescence, 1:1000 immunoblot) and anti-MAP2 (188002, Synaptic Systems, 1:500) anti-HA tag HRP conjugated (26183-HRP, Thermo Fisher Scientific, 1:1000), anti-FIP200 (10069-1-AP, proteintech,1:200) anti-WIPI2 (MCA5780GA, Biorad, 1:200). .. A rabbit polyclonal antibody targeting RyR2 was produced by LifeTein directed against the following epitope CKPEFNNHKDYAQEK of the mouse RyR2 sequence with a cysteine added to the N-terminal end (1:200) .



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    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
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    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
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    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
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    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
    Mouse Anti Lamp1 Antibodies, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Developmental Studies Hybridoma Bank rat monoclonal anti 1d4b lamp1
    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
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    Image Search Results


    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining

    ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining, Immunofluorescence, Marker